Trying to Login with DOMAIN\USERNAME but getting error “Login failed for user ‘DOMAIN\MACHINENAME$'”

There are two servers, one is app and one is Db both hosted on AWS separate EC2. App server have an IIS and logged in with service account to communicate with DB server. Application is using Trusted_Connection option in connection string so that not have to specify credentials and communicate as logged in user. While communicating App server hits with below error, IIS app was configured with default AppPoolIdentity –

System.Data.SqlClient.SqlException: Login failed for user ‘DOMAIN\MACHINENAME$’.

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“The page is performing an async postback but the ScriptManager.SupportsPartialRendering property is set to false. Ensure that the property is set to true during an async postback” OR “Invalid postback or callback argument”

Recently I was working on performance testing for one of my application and  came across this issue where all of my call failing in JMeter.

I started analyzing and found it was a wizard where series of calls were on same page using ASYNC Ajax calls which were complaining “The page is performing an async postback but the ScriptManager.SupportsPartialRendering property is set to false. Ensure that the property is set to true during an async postback”  due to which my main POST was failing with error “Invalid postback or callback argument”.

The strange thing was all of this was working fine when run via UI. Then started reading about when this error occurs and came across one discussion on a forum where they mentioned about SupportsPartialRendering is not supported by every browser

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